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General information Notification Number B/DK/55/Waxy Wotan Member State to which the notification was sent Denmark Date of acknowledgement from the Member State Competent Authority 22/02/2023 Title of the Project CrisprCAS modified potato with altered starch properties Proposed period of release: 01/05/2023 to 30/09/2023 Name of the Institute(s) or Company(ies) KMC Amba , Herningvej 60 Denmark 7330
Is the same GMPt been notified elsewhere by the same notifier? No Has the same GMPt been notified elsewhere by the same notifier? No Genetically modified plant Complete name of the recipient or parental plant(s):

Common Name	Family Name	Genus	Species	Subspecies	Cultivar/breeding line
potato	solanaceae	solanum	solanum tuberosum	tuberosum	Wotan

Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications: Waxy Wotan (K33)’The CrisprCAS modified potato, Waxy Wotan, Line K33, hereinafter 'Waxy Wotan (K33)', is generated at the University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C. Denmark.Using the CrisprCAS technology, small targeted mutations have been introduced in the Granular Bound Starch Synthase (StGBSS) 1 gene, which normally produces the GBSS1 enzyme, that forms the amylose component in starch. In Waxy Wotan (K33), gene function of the StGBSS1 gene (in all 4 alleles) is destroyed/non-functional, whereby the plant cannot produce amylose and therefore contains starch with only amylopectin. Genetic modification Type of genetic modification: Other; Other

CrisprCAS modification with targeted mutations on StGBSS 1 gene. Brief description of the method used for the genetic modification: The CrisprCAS based generation/breeding of Waxy Wotan (K33), incl description of transient plasmid-based CRISPR/SpCas9 construct, protocols for transformation of protoplast cells, regeneration of edited plant from single protoplast cell, screening and sequencing of mutations, is described in detail inJohansen IE, Liu Y, Jørgensen B, Nielsen KL, Andreasson E, Bennett EP, Nielsen KL, Blennow A, Petersen BL (2019) High efficacy full allelic CRISPR/Cas9 gene editing in tetraploid potato (2019) Sci Rep 9, 17715 https ://doi.org/10.1038/s41598-019-54126-w  If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination: Not applicable Experimental Release Purpose of the release: The purpose of the release is to test the properties of the Waxy Wotan in agricultural land.Purpose is also to see the amount of modified starch and the quality of the starch, expected as a "Clean Label" product, and the growth of the plant. Geographical location of the site: Denmark, Herning, Arnborg Size of the site (m2): Below 500 m2 Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release: N/A Environmental Impact and Risk Management Summary of the potential environmental impact from the release of the GMPts: Note especially if the introduced traits could directly or indirectly confer an increased selective advantage in natural environments; also explain any signifant expected environmental benefitsPotatotes are grown all over the Union and are used for consumption, french fries, chips, alcohol and starch produktion.Potatoes are not known as a colonizer of wild ecosystems (OECD 1997), and does not occur outside the field. Very rarely does potato waste plants occur in the cultivated landscape since tubers are destroyed during the next years tillage, use of herbicides and competition of subsequent crops. Brief description of any measures taken for the management of risks: Most modern potato varieties have low pollen fertility and the formation of potato berries and seeds is extremely low. According to the literature, the distance of the spread of potato pollen is maximum of ten meters. A safety distance of min.15 meters will be held. All flower buds from the CrisprCAS modified plants will be removed. Cleaning of machinery, tools, and transportation vehicles will be done after contact with the modified lines. The field will be checked, post-harvest for growth of surviving tubers. The field will not be used to plant potatoes again in 4 years and until at least one growing season without surviving potatoes is observed. Potential waste tubers will be documented and eliminated.Transport of harvested potatoes will take place by car or truck in sealed double bags. Transport will not take place with other cultivated potatoes. When transporting, documentation must be included, stating that it is GM and the name and telephone number of a contact person. Analyses of the material will be in KMCs premises and leftover crop residues will be destroyed by autoclaving or incineration. Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release: N/A Final report - European Commission administrative Information Consent given by the Member State Competent Authority: Yes
05/10/2023